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HE Huamei¹ , ZHA0 Yingxue², WU Linxiu¹, ZHOU Weihai³, LIU Xihua¹, ZHEN Hanshen⁴

( 1.Guangxi Health Seience College, Nanning 530023 ,China; 2. Jiangbin Hospital of Guangxi Zhuang AutonomousRegion, Nanning 530021 ,China; 3. Guangxi Kekang Technology Group Co., Ltd, Nanning 530001 ,China;4. Guangxi University of Traditional Chinese Medicine, Nanning 530001 ,China)

Abstract Objeetive 'To explore the elfeets and mechanisms of hirudin on human kidney-2 cell ( HK-2 cells) usingtranscriptomics techniques. Methods The CCK-8 method was used to deteet the viability of HK-2 cells. andtranscriptome sequencing was performed in the solvent control group and hirudin group. The gene expression levels indifferent samples were calculated using the RSEM method, and differential gene screening was performed using theDEseq2 method with a screening criterion of llog2FCl>1 and P<0.05. Subsequently, enrichment analysis was performed based on differentially expressed genes.Results The hirudin(5 mg/mL)had the best effect on enhancingthe viability of HK-2 cells. 'Transcriptomic studies found that compared with normal HK-2 cells, there were 1723genes were differentially expressed in HK-2 cells treated with hirudin. G0 and KEGG analvsis results showed that theregulation of inllammatory pathways was the main mechanism by which hirudin inereased HK-2 cels viabilityConclusion Hirudin inereases the viability of HK-2 cells, and its mechanism may be involved the regulation ofinflammatory pathways.

Keywords:hirudin:human kidney-2 cells; transeriptome

DOI:10.19296/j.cnki.1008-2409.2024-02-013

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