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YUAN Xi^1a,2 , L Shanbin^1a , QIN Lingshi^1a ,ZOU Jian^1a,LIANG Chengqin^2b ,ZHOU Xianli^1a,2

( 1. a. College of Intelligent Medicine and Biotechnology, b. College of Pharmacy, Guilin Medical University,Guilin 541199, China; 2. Key Laboratory of Biochemistry and Molecular Biology ,Guilin Medical University,Guilin 541199,China)

AbstractObjective To investigate the anti-human colorectal cancer cell activity of Cycas cycas triterpenoidsaponins Cypaliuruside K in viro, and to analyze the mechanism of anti-human colorectal cancer cell activity ofCypaliurusidek, Methods Cypaliuruside K, isolated from Cyclocarya paliurus, was used as an experimental drug tointervene SW480 tumor cells. The experiment was divided into control group and drug group, and the total protein ineach group was extracted for tandem mass spectrometry( TM'T') proteomic analysis. Results There were 402 differentially expressed proteins. Subcellular localization analysis showed that most of the differentially expressedproteins were located in mitochondria, cytoplasm and cell membrane. Gene ontology ( GO ) analysis showed thatdifferential proteins were mainly involved in DNA replication , protein synthesis and glucose metabolism. The analysisresults of Kyoto Eneyclopedia of Genes and Genomes ( KEEG) showed that differential proteins were mainly involvedin cell metabolism, genetic information transmission, biological system pathways and other related signalingpathways. Conclusion Based on the 8 selected proteins closely related to anti-human colorectal cancer cells.quantitative proteomics preliminatively reveals that Cypaliuruside K may increase the expressions of MAPK8SMAD2, PMAIP1, BBC3, JUN, MAPKl, NRAS and decrease the expressions of CYCS and achieves anti-SW480tumor cell effeet in vitro.

Keywords: Cyclocarya paliurus; Cypaliuruside K; colorectal cancer; proteomies; bioinformatics

DOI:10.19296/j.cnki.1008-2409.2024-02-008

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