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YANG Yizhuang, CHEN Xu,AI Rui, HOU Xinyue, WANG Juan

(College of Pharmacy, Guilin Medical University, Guilin 541199,China)

Abstract Objective 'To investigate the proteetive elfeets of leonurine( LE0 )on pyroptosis induced by oxidized low.density lipoprotein( ox-LDL) in THP-1-derived maerophages. Vethods M'T'T assay was used to detect the viability of'THP-1 macrophages/'THP-1-derived foam cells after treatment with diflerent coneentrations of LEO. 0il Red Ostaining was used to assess the intracellular lipid accumulation, meanwhile the contents of intracellular totalcholesterl('TC ), triglycerides ( TG ), interleukin-1B ( 1-1B), interleukin-18 ( IL-18), and lactate dehydrogenase ( LDH) in the cell supernatan were detected. Westerm blot was used to detect the expression level of proteins relatedto cellular pyrolysis. The level of reactive oxygen species ( ROS) was determined by flow cytometry. Results As theconeentration of LE0 inereased, there was a positive correlation between cell activity and LE0 concentration. LEOintervention decreased the intracellular 'TC and 'TG levels, and reduced the intracellular lipid accumulation. LEOdecreased the expression of cellular pyroptosis-related proteins, including NLRP3 ,N-GSDMD,Cleaved Caspase-1. Atthe same time, treatment with LE0 reversed the ox-LDL-induced inerease in ROS production. Conclusion LEOdecreases lipid accumulation in 'THP-1 macrophages induced by ox-LDL and inhibits the pyroptosis induced by ox-LDL in THP-1-derired macrophage.

Keywords:leonurine;macrophages;ox-LDL;pyroptosis

DOI:10.19296/j.cnki.1008-2409.2024-02-007

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